Vibrio sp. RC341 shares 2956 ORFs with V. mimicus MB-451 (82% of Vibrio sp. RC341), and Vibrio sp. RC586 shares 3048 ORFs with V. mimicus MB-451

(84% of Vibrio sp. RC586) (Figure 1). Vibrio sp. RC341 and Vibrio sp. RC586 share 2926 ORFs with each other (81% of ORFs in both genomes) (Figure 1). Figure 1 Venn diagrams showing ORFs shared by Vibrio sp. RC341, Vibrio sp. RC586, V. cholerae N16961, and V. mimicus MB-451. The number in the middle shows the https://www.selleckchem.com/products/Cyt387.html conserved number of ORFs shared by the three strains. The numbers show that there are ORFs unique to that strain or that there are ORFs shared. To determine average nucleotide identity (ANI) and average amino acid identity (AAI) between each genome, the average pairwise similarity between ORFs conserved click here between the compared genomes was calculated, following methods of Konstantinidis and Tiedje [18] and Konstantinidis et al. LY2874455 manufacturer [19]. In this approach, two genomes with an ANI >95% and AAI >96% belong to the same species, while those with ANI and AAI below these thresholds, comprise separate species [19, 20]. The ANI and AAI between Vibrio sp. RC586 and Vibrio sp. RC341 was 85 and 92%, respectively (see Additional files 4, 5, and 6). The ANIs between Vibrio sp. RC586 and individual V. cholerae ranged between 84 and 86%, while the ANI

between Vibrio sp. RC341 and V. cholerae ranged between 85 and 86% (see Additional files 4, 5, and 6). The AAIs between Vibrio sp. RC341 and individual V. cholerae genomes and Vibrio sp. RC341 and V. cholerae were 92% in all comparisons (data not shown). The ANIs between Vibrio sp. RC586 and V. mimicus MB-451 and VM223 were 88% and 87%, respectively, and 86% for Vibrio sp. RC341 and both V. mimicus genomes (see Additional files 4, 5, and 6). The AAI between Vibrio sp. RC341 and V. mimicus strains MB-451 and VM223 was 92% in both comparisons, while the AAI between Vibrio sp. RC586 and both V. mimicus strains was 93% (data not shown). The V. cholerae genomes had ANI >95% and AAI >96% and both V. mimicus strains a 98% ANI and AAI. The ANI for all V. cholerae

and both V. mimicus strains was 86%. Based on these data, it is concluded that Vibrio sp. RC341 and Vibrio sp. RC586 are, indeed, separate species, genetically distinct from V. mimicus and V. cholerae and from each other. Strains of interspecies comparisons shared <95% ANI and <96% AAI with members of other species Aurora Kinase included in this study, the threshold for species demarcation [19, 20], as applied to Vibrio, Burkholderia, Escherichia, Salmonella, and Shewanella spp. [21, 19, 22]. When Vibrio sp. RC341 and Vibrio sp. RC586 were compared with the more distantly related V. vulnificus and V. parahaemolyticus, Vibrio sp. RC586 showed 72 and 72% ANI and 73 and 73% AAI, respectively and Vibrio sp. RC341 73 and 72% ANI and 73 and 73% AAI with V. vulnificus and V. parahaemolyticus, respectively (see Additional files 4, 5, and 6). Furthermore, comparative analysis of the rpoB sequence demonstrates that Vibrio sp.