Using these sample files containing TRF lengths (peak values) in base pairs, this program enabled us to assign a species name to each TRF by comparing each TRF of a T-RFLP fingerprint separately with the library. TRFs with a peak height of less than 10% of the highest peak were excluded from the

analysis, since such peaks rarely corresponded with any of the species shown to be present by cloning [41]. Statistical analysis To compare rates of occurrence of events, ordinary risk ratios with 95% confidence SNX-5422 cost intervals were calculated, except for paired data, in case of which we calculated McNemar odds ratios with 95% confidence intervals. Statistical significance was accepted at the two-tailed α = 0.05 significance level. All analyses were performed

with the statistical software package SPSS version 15.0 (SPSS, Chicago, Illinois). Acknowledgements This study was funded by the Marguerite-Marie Delacroix Foundation, the Special Research find more Fund (BOF) of the Ghent University, and the Fund for Scientific Research Flanders (Belgium). The Marguerite-Marie Delacroix Foundation, the Special Research Fund (BOF) of the Ghent University, and the Fund for Scientific Research Flanders (Belgium) were not involved in the development of the study design, the collection, analysis, and interpretation of the data, in the writing this website of the report nor in the decision to submit the paper for publication. References 1. Sobel JD: Bacterial vaginosis. Annu Rev Med 2000, 51:349–56.CrossRefPubMed 2. Schwebke JR: Gynecologic consequences of bacterial vaginosis. Obstet Gynecol Clin North Am 2003, 30:685–94.CrossRefPubMed 3. Boris S, Barbés C: Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes

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