Two blinded pathologists compared this to histological quantification of parenchymal granulocytes using the marker CD15. Semi-quantitative grading of steatohepatitis (SH) (mild, moderate or severe) was also assessed. Contemporaneous liver/spleen 99mTc-nanocolloid scintigraphy, in which hepatic activity was
expressed relative to the spleen, was performed in a subset with SAH and an additional group with inactive alcohol Bortezomib purchase related cirrhosis (ARC). Results: Seventeen patients with SAH (14 male, median DF 52, range 33%ndash;155; 14 studied with 111In-oxine radi-olabeling and 3 with 111In-tropolone) and 7 with ARC (all male, median MELD 9, range 7.5%ndash;13) were recruited. The 24h/30min activity ratios in SAH positively correlated with CD15 biopsy quantification (r=0.62, p=0.023). SH grading demonstrated good inter-observer agreement (Κ=0.886, p<0.001) and 24h/30min ratios PD0325901 concentration were significantly higher in those with histologically severe vs moderate vs mild SH (3.85 ±1.12, 2.29 ±0.99 and 1.42 ±0.36, respectively; p=0.01). Imaging
appearances in the SAH cohort receiving 111In-tropolone-labeled leukocytes were similar to the group studied with 111In-oxine. 99mTc-nanocolloid liver/spleen ratios were significantly lower in SAH (n=11) vs ARC (n=7; 0.80 ±0.59 vs 2.29 ±1.60, p=0.049) and did not correlate with histological CD15 quantification or SH grading. Conclusions: The 24h/30min hepatic activity ratio correlates with the histological severity of SAH, thereby validating 111 In-leukocyte click here scintigraphy as a non-invasive diagnostic tool in this condition. Additional corroborative data from 99mTc-nanocolloid scintigraphy suggest that liver activity in
111In-leukocyte scintigraphy reflects active neutrophil migration rather than physiological destruction of neutrophils or non-specific phagocytosis of free 111 In. Disclosures: Sumita Verma – Advisory Committees or Review Panels: Janssen; Grant/Research Support: Gilead, Roche, Janssen The following people have nothing to disclose: Jonathan R. Potts, Mark Howard, Mark Taylor, Neda Farahi, Sarah Heard, Arun N. Shankar, Graeme J. Alexander, Edwin R. Chilvers, Adrien M. Peters “
“As an important epigenetic mechanism, histone acetylation modulates the transcription of many genes and plays important roles in hepatocellular carcinoma (HCC). Aberrations in histone acetylation have been observed in HCC, but the factors that contribute to the aberrations have not been fully elucidated. MicroRNAs (miRNAs), which are noncoding RNAs that regulate gene expression, are involved in important epigenetic mechanisms. In this study, we determined that miR-200a and the level of histone H3 acetylation at its promoter were reduced in human HCC tissues in comparison with adjacent noncancerous hepatic tissues.