This indicates that curcumin may further dampen microglial activation by interfering with two other key transcription factors expressed in activated microglial cells. In addition to its Lapatinib order inhibitory effects on pro inflam matory signaling, two well known anti inflammatory molecules, PPARa and IL4, were significantly induced by curcumin. PPARa and IL4 both specifically Inhibitors,Modulators,Libraries inhibit pro inflammatory activation of microglial cells and some of the immune dampening effects of curcumin may be mediated via this signaling axis. The cell culture experiments with conditioned media from BV 2 cells showed that curcumin significantly reduced LPS triggered microglial neurotoxicity on 661W photoreceptor Inhibitors,Modulators,Libraries cells. We hypothesize that the strong sup pression of LPS induced Nos2 transcription by curcumin is a major pathway responsible for this phenomenon.
In this context, Mandal et al. have recently demonstrated that curcumin protects 661W cells from hydrogen Inhibitors,Modulators,Libraries perox ide induced cell death. This effect is very likely mediated by the antioxidant and radical scavenging capa city of curcumin. In a model of light induced retinal degeneration, curcumin also suppressed inflammatory marker expression in vivo, which could be potentially mediated by its attenuating effect on retinal microglia. Conclusions We have shown that curcumin triggered global changes in the transcriptome of resting and LPS activated micro glial cells. In addition to its known function in blocking pro inflammatory gene expression via interference with NFkB signaling, curcumin induced novel anti inflamma tory targets in microglia.
Curcumin also significantly inhibited microglial migration and cytotoxicity, which are key features of neuroinflammation. Our publicily avialable dataset provides a basis to understand the pleiotropic beneficial effects of curcumin on microglia as key innate immune cells of the nervous system. Moreover, the results of this study also underscore the importance Inhibitors,Modulators,Libraries of curcumin as a promising Inhibitors,Modulators,Libraries dietary com pound for the treatment of various neurodegenerative disorders Nutlin-3a CAS associated with inflammation. Background The blood brain barrier is a selective barrier that is created by the endothelial cells in cerebral microvessels. Endothelial cells and supporting cells such as astrocytes, pericytes, neurons, and perivascular microglia are orga nized together to form the neurovascular unit which is essential for induction, function, and support of the BBB. In contrast to the considerable knowledge character izing the crosstalk among brain endothelial cells, astro cytes, and microglia within the neurovascular unit during inflammation, very little is known about the role played by the brain microvascular pericyte.