The extremely high absorption of MM could be due to the easy penetration of the small structures into fabrics. However, the increase in the size of these structures (see Table 3) did not prevent their exit from the fibres, and desorption was notable. This finding could be due to the higher permeability of textiles compared with human skin, which may explain why this effect was not observed [25, 26]. To study the penetration of active principles through the skin, an in vitro methodology based on percutaneous absorption

is performed to demonstrate the delivery of an encapsulated principle from a textile to the ABT-888 mw different layers of the skin (stratum corneum, epidermis, or dermis). The percutaneous Inhibitors,research,lifescience,medical absorption of the two formulations, Lip (2% GA, 4% PC) and MM (2% GA, 4% PC, and 30% Oramix CG 110), was evaluated, as were the CO and PA textiles impregnated

with the same Lip or MM. The two formulations and Inhibitors,research,lifescience,medical the CO and PA textiles previously treated with the Inhibitors,research,lifescience,medical formulations were placed in contact with the skin discs as described in Section 2. The aim of this assay was to demonstrate tracer delivery into the different layers of the skin. GAs encapsulated in MMs and Lips, which were either embedded or not embedded in cosmetotextiles, were applied to the skin to study the percutaneous absorption profiles of the agents. The GA extracted from a washing sample, the fabric, the stratum corneum, the rest of the epidermis, the dermis, and the receptor Inhibitors,research,lifescience,medical fluid was analysed. The results are listed in Table 4 and graphically represented in Figure 2. Figure 2 In vitro percutaneous absorption of gallic acid (GA) in Lip and MM formulations and the PA and CO cosmetotextiles Inhibitors,research,lifescience,medical (SC: stratum corneum, R. Fluid: receptor fluid) (significant level accepted *P < 0.01). Table 4 In vitro percutaneous absorption

of GA (gallic acid) in Lip and MM formulations and the PA and CO cosmetotextiles. Comparison of percutaneous absorption in percentage indicates that it is higher when GA was applied as a formulation (Lip or MM) than when it is applied through cosmetotextile. Besides, CO delivers to the skin GA in a greater extent than PA. As shown in Figure 2, the penetration of GA formulated in Lip nearly was much higher than that of GA formulated in MM. All skin compartments showed a higher amount of GA when vehiculised with Lip than when vehiculised with MM. This result could be due to the bilayer structure of the Lip, which is similar to the lipid bilayer structures present in the SC and in the cellular membranes of the skin [28]. Evidence that Lips do not penetrate deeper than the stratum corneum layer has been published [29]. However, Lips enhance the penetration of both hydrophilic and lipophilic drugs [30, 31].