Specimens were stained for nitric oxide synthase 2 (NOS2) (also denoted iNOS) as a marker for the M1 macrophage

phenotype and the scavenger receptor CD163 as a marker for the M2 macrophage phenotype. The expression level of COX-2 was examined both by immunohistochemistry and western blot. Results: Stroma of Apc (Min/+)mouse polyps was infiltrated by TAMs that were premodinantly polarized to M2 phenotypes. Berberine reduced the size and number of polyps, and also reduced the number of F4/80 positive macrophages. Concomitantly, Ceritinib cell line the expression of iNOS was significantly upregulated and the expression of COX-2 was decreased by berberine, although the expression of CD163 were not significantly altered. Conclusion: Berberine inhibits the growth of polyps and alters the phenotype of TAMs in stroma of Apc (Min/+)mouse polyps possibly through inhibiting the expression of COX-2. Key Word(s): 1. Berberine; 2. Apc (Min/+) mouse; 3. TAMs; 4. COX-2; Presenting Author: YU-MING WANG Additional Authors: YING CHANG, YUAN-YUAN CHANG, JING CHENG, JING LI, TAO WANG, QING-YU ZHANG, DONG-CHUN LIAJNG, BEI Atezolizumab purchase SUN, BANG-MAO WANG Corresponding

Author: YU-MING WANG Affiliations: Tianjin Medical University General Hospital Objective: To evaluated the links between the serotonin transporter gene polymorphism and SERT expression levels. We examined SERT mRNA, SERT protein levels as well as the ultrastructure in colon biopsies from patients with different 5-HTTLPR genotypes. Methods: Two hundred and fifty-four patients with IBS and 120 healthy subjects were studied. DNA 上海皓元医药股份有限公司 samples were extracted from

peripheral blood and genotyped by polymerase chain reaction. SERT mRNA and protein levels were evaluated by quantitative real time PCR and western blotting. The promoter efficiency of the serotonin transporter promoter was evaluated with luciferase reporter system. The ultrastructures of colon were performed by means of cryogenic transmission electron microscopy. Results: The frequency of the L/L genotype in C-IBS group was significantly higher than that in the control and D-IBS. However, the S/S genotype in D-IBS was significantly higher than that in C-IBS. The transcriptional efficiency of the L/L genotype was significantly higher than that in the L/S and S/S genotype. Patients with the L/L genotype demonstrated increased production of the SERT protein when compared with L/S and S/S patients. The l variant increased SERT promoter activity by 2.43-fold when compared with the s variant. The caliciform cells dense of D-IBS were higher than that of A-IBS and C-IBS. The intra-cellular mucocysts were significantly increase and productive. The neuroendocrine cells were grow in number, and were full of electron-dense secretory granules which were casted off to mesenchymal on occasion. The cell population of plasmocytes were increased in C-IBS. The labrocytes with electron-dense secretory granules and a few vacuolus were significantly increased in mesenchymal of colonic mucosa in D-IBS.