Phytopathology 1980, 70:712–715.CrossRef 36. Kovach ME, Elzer PH, Steven Hill D, Robertson GT, Farris MA, Roop Ii RM, Peterson KM: Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995,166(1):175–176.CrossRefPubMed 37. Spaink HP, Okker RJH, Wijffelman CA, Pees E, Lugtenberg BJJ: Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol Biol 1987,9(1):27–39.CrossRefPubMed

38. Sambrook J, Russel DW: Molecular https://www.selleckchem.com/products/PD-0325901.html Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001. 39. de Bruijn I, de Kock MJD, de Waard P, van Beek TA, selleck compound Raaijmakers JM: Massetolide A biosynthesis in Pseudomonas fluorescens . J Bacteriol 2008,190(8):2777–2789.PubMedCentralCrossRefPubMed find more 40. de Bruijn I, Raaijmakers JM: Regulation of cyclic lipopeptide biosynthesis in Pseudomonas fluorescens by the ClpP protease. J Bacteriol 2009,191(6):1910–1923.PubMedCentralCrossRefPubMed 41. Miller JH: Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1972. 42. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 1990,215(3):403–410.PubMed 43. Bachmann BO, Ravel J: Chapter 8 methods for in silico prediction of microbial polyketide and

Selleckchem Lumacaftor nonribosomal peptide biosynthetic pathways from DNA sequence data. In Method Enzymol. Edited by: David AH. PA: Academic Press; 2009:181–217. vol. Volume 458 44. de Bruijn I, de Kock MJD, Yang M, de Waard P, van Beek TA, Raaijmakers JM: Genome-based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species. Mol Microbiol 2007,63(2):417–428.CrossRefPubMed 45. Tamura K, Peterson D, Peterson N, Stecher G, Nei

M, Kumar S: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011,28(10):2731–2739.PubMedCentralCrossRefPubMed 46. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B: Artemis: sequence visualization and annotation. Bioinformatics 2000,16(10):944–945.CrossRefPubMed 47. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J: ACT: the Artemis comparison tool. Bioinformatics 2005,21(16):3422–3423.CrossRefPubMed 48. Abbott JC, Aanensen DM, Rutherford K, Butcher S, Spratt BG: WebACT-an online companion for the Artemis Comparison Tool. Bioinformatics 2005,21(18):3665–3666.CrossRefPubMed 49. Blumer C, Heeb S, Pessi G, Haas D: Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proc Natl Acad Sci USA 1999,96(24):14073–14078.PubMedCentralCrossRefPubMed 50.