Following the completion of the dynamic warm-up protocol the subjects were then led to the laboratory for agility selleck chem testing. Heavy resistance warm-up treatment (HRW) The HRW treatment consisted of a general warm-up of five minutes jogging around a 200 meter indoor track, followed by three sets of parallel back squats; five squats with a load equivalent to 50% 1-RM, three squats with a load equivalent to 60% 1-RM, and three squats with a load equivalent to 90% 1-RM. A two minute recovery period was allowed between each set of squats. After the completion of the heavy resistance warm-up protocol the subjects were then led to the laboratory for agility testing. Both warm-up treatments were designed following the guidelines suggested by Baechle et al.

(2008), consisting of a general phase (5 min of jogging) and a specific phase (DW or HRW treatment). Between the two treatments, the only variation was to the specific phase where subjects performed either the DW or HRW protocol. Agility shuttle test Subjects performed three 10 m agility shuttle tests (Figure 2). The test was modified from a standard 20 m (5m-10m-5m) shuttle test to a shorter distance due to lack of sufficient laboratory space. The agility trials were performed at four, eight, and twelve minutes post HRW or DW treatments. Times of the agility tests were recorded using a dual-beam photocell infrared timing system (Swift Performance Equipment, Lismore, Australia). Figure 2 Diagram of the 10m agility shuttle test. The arrows show the path followed by a subject cutting to the right.

The 10 m agility shuttle test was selected due to its specificity to change of direction maneuvers used in the sports of tennis and basketball. Subjects started the test by stepping from a 0.30 m box and sprinting 2.5 m in their preferred direction. This initial sprint direction was established during the familiarization session and remained consistent throughout all tests. Subjects starting to the right direction stepped off the box to the left of the start/finish line and subjects starting to the left stepped off the box to the right of the start/finish line (Figure 2). Stepping from a box was selected in order to maximize the role of reactive strength and stiffness at the start of the test. This also helped replicate the movements specific to actual game play e.g., landing from a shot or jump and then sprinting with changes of direction.

The reliability of the time for the modified agility shuttle Carfilzomib test was established by using the subjects times during each of the trials following the DW treatment. The intra-class correlation was 0.96 with a 95% confidence interval (CI) = 0.88�C0.99, while the coefficient of variation was 2.8% with the 95% CI = 2.1�C4.4%. An eight camera motion analysis system (Vicon?, Oxford, UK) was used to capture the maneuvers performed by the subjects during the agility shuttle test. The system sampled at 100 Hz.