Finally, in sections of injured sciatic nerves, we analyzed the expression of Cdc2 and GAP-43 proteins that are both up-regulated during peripheral regenerative processes. Compared to mice subjected to long-lasting
treadmill running, mice subjected to short-lasting treadmill running showed an acceleration of the regenerative processes at the injured sciatic nerve. Our data demonstrate that short-lasting treadmill running, by reducing the neuropathic pain symptoms and facilitating the ISRIB molecular weight regenerative processes of the injured nerve, have beneficial rehabilitative effects on the functional recovery after peripheral nerve injury. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.”
“Aims:
To verify the specificity of a PCR assay for the identification and diagnosis of Edwardsiella ictaluri.
Methods and Results:
An Edwardsiella ictaluri-specific PCR assay was developed utilizing two features of the ribosomal DNA gene clusters. The first feature is the presence of two ribosomal gene clusters located in tandem to one another (the inter-ribosomal spacer, IRS). This characteristic is present in the Edwardsiella
genus but absent in the other sequenced members of the Enterobacteriaceae. The second feature is the presence of an intervening sequence (IVS) in the 23S rRNA gene of Edw. ictaluri. To verify the specificity of this assay, we tested genomic DNA TPX-0005 from a variety of bacterial species. The IVS/IRS PCR assay results in an c. 2000-bp product from all Edw. ictaluri isolates tested, but not from any other species including Edwardsiella tarda.
Conclusions:
The IVS/IRS PCR assay is highly specific for Edw. ictaluri and useful as a tool for identifying this pathogen.
Significance and Impact of the Study:
This research verifies the specificity of PCR-based assay for Edw. Ictaluri, and we describe this assay as a highly
versatile diagnostic tool for its identification.”
“Aspirin, whose active ingredient is sodium salicylate, is the most widely used drug worldwide, but it is not recommended for children because it may cause Reye’s syndrome. High doses old of salicylate also induce temporary hearing loss and tinnitus; while these disorders are believed to disappear when treatment is discontinued some data suggest that prolonged treatment may be neurotoxic. To investigate its ototoxicity, immature, postnatal day 3 rat cochlear organotypic cultures were treated with salicylate. Salicylate did not damage the sensory hair cells, but instead damaged the spiral ganglion neurons (SGN) and their peripheral fibers in a dose-dependent manner. The cross-sectional area of SGN decreased from 205 mu m(2) in controls to 143, 116, and 91 mu m(2) in cultures treated with 1, 3, or 5 mM salicylate, respectively.