Among these noble

metal plasmonic nanoparticles, gold this website nanorods (GNR) in particular, PRIMA-1MET order with its varied size, low reactivity, unique anisotropy shape, and optical properties, have been widely investigated by many research groups [1–3]. On the other hand, the LSPR frequency shifting has been widely used in chemical, gas [4] and bio-sensors [5], to examine the chirality of molecules [6] and be used as an electromagnetic energy transmitter [7] based on various types of pure- [8] or modified-metallic nanostructure array on glass substrate or nanoparticles in bulk solution [9]. In fact, developing of nanoparticle-based sensing materials is important and urgent for detection in special environment, for example, detection of single

molecule MDV3100 solubility dmso analyte of internal cell [10–12]. The free-label or monolayer/functionalized nanosensors have been achieved by fluorescence protein [13, 14], polymer [15, 16], quantum dots (QDs) [17], graphene oxide [18], and metal nanoparticles [19] through monitoring the variations in their fluorescence intensity or lifetime. However, the intrinsic drawbacks of fluorescence probe are photo-bleaching and blinking [20]. Furthermore, the cytotoxicity of the QDs makes them practically useless for in vivo biological application. Therefore, it is an urgent task to develop biocompatible and highly photostable nanoparticles for nanosensors, in particular, based on the extinction/scattering, and therefore, with non-blinking is highly preferential. Recently, Zijlstra et al. have demonstrated a label-free optical detection of single non-absorbing molecules by monitoring the plasmon resonance of nanorod via a sensitive photothermal spectra [21].

Generally speaking, optical sensors of metallic nanoparticles can be achieved by exploiting the sensitivity to local refractive index (n) of the surrounding medium (Δλ max ≈ Δn) or to the plasmon band shift that is caused by the proximity of nanoparticles [21–24]. In this study, we investigate the pH-dependent local surface plasmon shift in a functionalized GNR. The gold Rucaparib in vivo nanorods modified by 11-mercaptoundecanoic acid (GNR-MUA) exhibit excellent stability and are easy to prepare, therefore can be the outstanding potential candidate for nanosensors. More importantly, it is based on the extinction spectrum (scattering) and thus non-blinking. We verified this optical signal originates neither from the aggregation of nanorods nor the variation of refractivity index through ion strength test and the pH titration procedure by comparing a modified pH-independent molecule (1-undecanethiol (UDT)) with MUA. We speculate that the dipole moment changes of MUA ligands on a rod surface play a very important role in this nanoparticle based-sensing system.