7% (2/12) 0/2 ST398 13.3% (2/15) 0/2 ST59 11.8% (2/17) 2/0 ST5 10.9% (20/184) 100.0% (20/20) ST7 7.4% (2/27) 0/2 ST680 5.6% (1/18) 1/0 ST188 4.8% (1/21) 0/1 ST239 3.5% (7/202) 7/0 ST1036 1/2 0/1 ST121 1/1 0/1 a STs with less than 10 isolates were not calculated in the percentage of genes present or MRSA/MSSA. The prevalence of different genotypes in different wards To investigate whether there were epidemic S. aureus clones that could survive and spread in different wards, we next analyzed the ICU, one of the largest

comprehensive Fer-1 datasheet surgical wards, and two of the largest medical wards. As shown in Figure 3, different STs were detected in different wards, and each ward had its own dominant STs. ST239 was a robust sequence type, and was prevalent in the ICU and surgical ward, while ST5 was prevalent in both medical wards and surgical wards. In medical wards, ST5, ST1, and ST680 were the predominant three clones, whilst isolates belonging to other STs were recovered at a rate of three isolates per month. Pulsed-field gel electrophoresis (PFGE)

was used to compare the genetic variation of the dominant STs recovered from different wards. Figure 3 (E and F) showed that the restriction profiles of the same epidemic S. aureus clones originating from the same wards were not identical. The major DNA restriction pattern was named type A, and isolates with closely (1–3 fragment TPCA-1 molecular weight differences) or possibly related (4–6 fragment differences) Edoxaban restriction patterns were considered subtypes of A, and were designated type A1, type A2, and so on. Those with more than six fragment differences were regarded as type B [13]. PFGE type A1 was the major pattern

of the prevalent clone ST239 in the ICU, but the PFGE patterns of prevalent clone ST5 in medical ward 1 were more dispersed. Figure 3 Dynamic changes of the epidemic S. aureus clones in different wards in 2011. A-D: Dynamic changes of the top five most prevalent S. aureus clones in the ICU (A), the largest comprehensive surgical ward (B), and two large medical wards (C and D). E-F: PFGE profiles of the dominant STs recovered from the same wards. The PFGE profiles of ST239 recovered from the ICU (E). The PFGE profiles of ST5 recovered from medical ward 1 (F). The major DNA restriction pattern was named type A, and isolates with closely (1–3 fragment differences) or possibly related (4–6 fragment differences) restriction patterns were considered subtypes of A, and were designated type A1, type A2, and so on. Those with more than six fragment differences were regarded as type B. Discussion Surveillance data from China suggested that S. aureus infections account for a substantial burden of disease [6]. Most of the individuals infected with hospital-onset S. aureus in this study were men (66.0%), which was consistent with findings from a previous study [14]. Unlike the incidence of community-onset S. aureus, which is highest in the younger age MK-8931 cell line groups [15, 16], hospital-acquired S.