4% and 46.1%, respectively. Conversely, monounsaturated FAs were the major constituents of CF and swine tissue, 38.7% and 40.3%, respectively. Our results strongly suggest that host-derived FAs might translocate across
the parasite syncytial membrane and be stored in the CF. (C) 2012 Elsevier Ireland Ltd. All rights reserved.”
“Objectives To evaluate whether the measurement of maternal serum inhibin A, activin A and placental growth factor (PlGF) at 12 + 0 to 16 + 0 weeks of gestation alone or in combination with second-trimester uterine artery Doppler pulsatility index (PI) is useful in predicting pre-eclampsia.\n\nMethods This was a case-control study of pre-eclampsia. From pregnant women attending their first antenatal examination at 12-16 weeks we collected serum samples and stored them at -80 degrees C. All patients ARS-1620 also underwent uterine
artery Doppler examination to measure the PI at 22-24 weeks’ gestation. We retrieved for analysis frozen samples from women who then developed pre-eclampsia, as well as three control samples per woman, matched for gestational age and storage time. Inhibin A, activin A and PlGF were measured using an enzyme-linked immunosorbent assay (ELISA) by an examiner who was blinded PF-562271 inhibitor to the pregnancy outcome.\n\nResults There were 31 cases with pre-eclampsia and 93 controls. Second-trimester uterine artery PI and marker levels were expressed as multiples of the median (MoM). The uterine artery PI was increased in pregnancies with pre-eclampsia compared with controls (mean +/- SD, 1.45 +/- 0.31 MoM vs. 1.02 +/- 0.25 MoM, P < 0.001), as were the level of inhibin A (mean +/- SD, 1.57 +/- 0.34 MoM vs. 1.08 +/- 0.43 MoM, P < 0.001) and the level of PI3K inhibitor activin A (mean +/- SD, 1.68 +/- 0.38 MoM vs. 1.06 +/- 0.42 MoM, P < 0.001). The level of PlGF was decreased in pre-eclampsia compared with controls (mean +/- SD, 0.69 +/- 0.23 MoM vs. 1.00 +/- 0.26 MoM, P < 0.001).
Receiver-operating characteristics curves were analyzed for controls and cases and areas under the curve (AUC) were 0.796 (95% CI, 0.712-0.880, P < 0.001) for inhibin A, 0.823 (95% CI, 0.746-0.899, P < 0.001) for activin A, 0.831 (95% CI, 0.752-0.910, P < 0.001) for PlGF and 0.851 (95% CI, 0.783-0.920, P < 0.001) for uterine artery PI. The combination of activin A, inhibin A and PI using logistic regression analysis yielded an AUC of 0.907 (95% CI, 0.830-0.938, P < 0.001) with a sensitivity of 87% and a specificity of 80%. The combination of activin A, PlGF and PI gave an AUC of 0.925 (95% CI, 0.852-0.978, P < 0.001) with a sensitivity of 90% and a specificity of 80%. Combining all four markers gave an AUC of 0.941 (95% CI, 0.891-0.990, P < 0.001) with a sensitivity of 93% and a specificity of 80%.