Table 1 A summary of CoBaltDB precomputed features-tools Program Reference Analytical method CoBaltDB features prediction group(s) LipoP

1.0 Server [59] HMM + NN LIPO   SEC     DOLOP [57] RE LIPO         LIPO [56] RE LIPO         TatP 1.0 [53] RE + NN   TAT       TATFIND 1.4 [52] RE   TAT       PrediSi [112] Position weight matrix     SEC     SignalP 3.0 Server [45–47] HMM + NN     SEC     SOSUIsignal [113] Multi-programs     SEC     SIG-Pred J.R. Bradford Matrix     SEC     RPSP [44] NN     SEC     Phobius [48, 49] HMM     SEC αTMB   HMMTOP [71] HMM       αTMB   TMHMM Server v.2.0 [70] HMM       #Mocetinostat mouse randurls[1|1|,|CHEM1|]# αTMB   TM-Finder [65] AA FEATURES       αTMB   SOSUI [114] AA FEATURES       αTMB   SVMtm [73] SVM       αTMB  

SPLIT 4.0 Server [115] AA FEATURES       αTMB   MCMBB [116] HMM         βBarrel TMBETADISC: [117]             _COMP   AA FEATURES         βBarrel _DIPEPTIDE   Dipeptide composition         βBarrel _MOTIF   Motif(s)         βBarrel TMB-Hunt2 [118] SVM         βBarrel HMM: Hidden Markov Model, NN: Neural Network, RE: Regular Expression, AA: Amino Acid, SVM: Support Vector Machine Table 2 A summary of CoBaltDB precomputed meta-tools Program Reference Analytical method YH25448 ic50 Localizations Subcell Specialization Server 2.5 [119] Multiple classifiers 5 diderms/3 monoderms SLP-Local [120] SVM 3 with no distinction SubLoc v1.0 [121] SVM 3 with no distinction Subcell (Adaboost method) [122] AdaBoost algorithm 3 with no distinction SOSUIGramN [123] Rolziracetam Physico-chemical parameters 5 diderms/no monoderm SVM: Support Vector Machine Table

3 A summary of CoBaltDB integrated databases and tools features. Databases Reference Features predicted Genome(s) Protein numbers EchoLOCATION [124] Subcellular-location (EXP) E. coli K-12 4330 (506 exp) Ecce _ Subcellular-location E. coli K-12 306 LocateP DataBase [89] Subcellular-location 178 MD 542788 cPSORTdb [91] Subcellular-location 140 BA 1634278 ePSORTdb [91] Subcellular-location (EXP)   2165 THGS [125] Transmembrane Helices 689 PROK 465411 Augur [88] Subcellular-location 126 MD 111223 CW-PRED [126] Cell-anchored (surface) 94 MD 954 PROFtmb [78] Beta-barrel (OM) 78 DD/19 MD 2152 HHomp [127] Beta-barrel (OM)   12495 PRED-LIPO [58] Lipoprotein SPs 179 MD 895 SPdb [90] Signal peptides (SPs) 855 PROK 7062 ExProt [128] Signal peptides (SPs) 23 AR/61 MD/115DD   Signal Peptide Website _ Signal peptides (SPs) 384 BA 1161 (EXP) PRED-SIGNAL [129] Signal peptides (SPs) 48 AR 9437 TMPDB [130] Alpha Helices & Beta-barrel   188 DTTSS Shandong Univ.