dipsaci populations obtained in our study shared a 99–100% identity with Roxadustat datasheet each other as well as with other D. dipsaci populations deposited previously in databases. The

only population (S) isolated from V. faba spp. minor was identified as D. gigas. Comparison of the nucleotide sequence of this population revealed a 99% identity with other D. gigas populations described so far. The populations D8, 1 and 2, of D. destructor, compared with other populations of this species present in GenBank, showed an identity level between 68.5 and 99.8%. American and Chinese populations described as haplotype C (Subbotin et al. 2011) showed the highest identity level (99.0–99.8%) with the D8, 1 and 2, of D. destructor populations. Polish populations of D. destructor analysed share only a 93% identity with the Polish population (Stu3), described previously (Marek et al. 2010). The phylogenetic analysis for D. dipsaci revealed a phylogenetic tree which was similar to that obtained by Subbotin et al. (2005). Two separate clades for diploid races and polyploidy

races were indicated (Fig. 1). It is important to note that when looking at the tree topology, populations isolated from the same host are rarely grouped together. This can be observed (e.g. for D. dipsaci populations isolated from Allium cepa or Cichorium spp.), and it cannot be explained PF-02341066 research buy by host or geographic origin. In the case of D. dipsaci, there are more than 30 distinguished host races that are supposed to be at different stages of speciation; however, some authors indicate that there is ambiguity about how they Cyclin-dependent kinase 3 should be defined (Sturhan and Brzeski 1991). Phylogenetic analysis

for D. destructor populations was performed for populations isolated from S. tuberosum, I. batatas and Astragalus mongholicus. For D. destructor, length variability of the ITS1 fragment was found, and eight haplotypes were separated (Subbotin et al. 2011). The haplotype A isolated from sweet potato from China is the most distinct and formed a separate clade. The previously reported population (Stu3) from Poland was assigned as haplotype G (Marek et al. 2010; Subbotin et al. 2011). The populations described in this study (D8, 1, 2), however, grouped together with haplotype C populations on a phylogenetic tree (Fig. 2). This indicates that in Poland, there are at least two haplotypes present. It is worth noting that most populations isolated from the sweet potato cluster separately from those isolated from potato. But at the same cluster with the presently described D. destructor populations from Poland grouping members of haplotype C, there is also a Chinese population from I. batatas (Fig. 2). Phylogenetic analysis was carried out with D. gigas found on V. faba minor seeds in Poland. In spite of a very high identity level with other populations reported so far from Europe and Northern Africa, D. gigas from Poland grouped separately from all of them.