As the difference between the logarithms of two values is the logarithm of the ratio of these values, we interpreted the meaning of the regression parameters as the percentage increase of the titre per given unit (for continuous factors) or compared to the reference category (for categorical factors). A multivariate logistic model was further developed to analyse the association between given variables and an observed increase in HIV RNA levels between sera obtained ‘PRE’ and

‘POST’ dose [an ‘increase’ was defined as HIV RNA <20 copies/mL at baseline (PRE) and HIV RNA >20 copies/mL after the two immunization doses (POST)]. In addition, clinically significant variables, such as CD4 cell count (<350 and >500 cells/μL) and time since HIV infection, were introduced into the model. The significance Selleck RG7204 Acalabrutinib chemical structure level was defined as 0.05. Data were analysed using s-plus 8.0 (Insightful Corp., Seattle, WA). The clinical characteristics of the 121 HIV-infected patients and 138 healthy controls are described in Table 1. In comparison with the healthy controls, the HIV-infected population included a higher percentage of male (68.6 vs. 42.8% in the controls; P = 0.0001) and non-Caucasian (40.5 vs. 16.7% in the controls; P < 0.0001) participants. The median age of HIV-infected patients was lower than that of the controls (median 46.4 vs. 50.9

years, respectively; P = 0.0005), which was explained by the lower proportion of HIV-positive individuals above 60

years of age (9.9 vs. 28.3%, respectively). As the inclusion criteria for HIV-infected patients required either a very high or a very low CD4 T-cell count, differences in median CD4 cell count, CD4 cell count nadir and disease severity between these two subgroups were significant, as expected (CDC category; Table 1). At the time of enrolment, one-third of HIV-positive individuals with a CD4 count <350 cells/μL had been diagnosed with AIDS. Most patients (108 of 121; 89.3%) Sorafenib manufacturer were being treated with antiretroviral drugs and baseline HIV RNA levels were below the detection level in 88 of 121 patients (72.7%). More HIV-positive patients than healthy subjects had been previously immunized against seasonal influenza (P < 0.0001), in accordance with Swiss recommendations. Five HIV-positive patients declined the second vaccine dose and one left the study area, while 11 HIV-infected individuals and seven healthy subjects did not present themselves to the second study appointment and remained unreachable after three phone calls. Altogether, 104 of 121 (86.0%) HIV-positive patients and 131 of 138 (94.9%) healthy subjects completed enrolment and were included in the final analysis of vaccine antibody responses. During the following season of 2010/2011, 66 of the originally 121 patients (54.5%) agreed to participate in the follow-up study and provide plasma samples prior to and following one dose of nonadjuvanted trivalent seasonal influenza vaccine.