5% (53/559) and 6.3% (13/207) of episodes were identified as severe by the CSS (≥17) (Fisher’s Exact, p ≤ 0.001) ( Table 4). This pattern remained across sites, gender and age group. The results in Table 5 demonstrate poor agreement in categorizing severe gastroenteritis between the two scoring systems when using the original severity classifications, but that agreement improves substantially when using modified severity classifications. When using the original scoring classification, every episode categorized as severe according to the CSS was also classified as severe according to the VSS; 76.7% (174/227) and 88.8% (103/227) of severe VSS in Africa and Asia, respectively,

RG7204 price were identified as not severe according to the CSS. When a modified scoring classification based on the mean scores (VSS: ≥10 Africa, ≥11 Asia; CSS: Africa and Asia ≥10) is used, the proportion of severe VSS cases classified MLN2238 as not severe by the CSS was reduced to 17.1% (49/287) in Africa and to 9.5% (11/116) in Asia, with 14.7% and 9.5% of CSS severe cases in Africa and Asia, respectively, classified as not severe according to the VSS. As compared to the original classification, when the modified scoring classification based on a threshold set at the median of the scoring distribution

(VSS: ≥11; CSS ≥13) was used, the proportion of severe VSS cases classified as not severe by the CSS was reduced to 35.7% (81/227) in Africa and 48.3% (56/116) in Asia, with 5.8% (9/155) and 3.2% (2/62) of CSS severe cases in Africa and Asia, respectively, classified as not severe according

to the VSS. Notably, while there were still differences in severe gastroenteritis categories when using either of the modified classifications, the agreement between the two scoring systems improves substantially as compared to the original severity classification; from kappa = 0.27 and kappa = 0.10 in Africa and Asia using the original severity classifications Calpain to kappa = 0.68 and kappa = 0.78 using the mean score modified classification and kappa = 0.65 and kappa = 0.47 using the median of the scoring distribution modified classification. In these randomized, controlled efficacy trials of PRV in low-resource settings in Africa and Asia, the VSS and CSS performed differently, with the VSS classifying more cases as severe in both regions. Using the VSS as compared to the CSS resulted in approximately four and nine times the number of severe cases in Africa and Asia, respectively ( Table 4). These results are consistent with those identified by Givon-Lavi et al. [23] in a study conducted using a different design – a prospective hospital-based observational study – and among a different population – children less than 5 years of age in Israel.